AMOLF Store for Science - Optical Tweezers

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Optical Tweezers

Description

Instrument integrates multiple (time-shared) optical tweezers with DIC high-resolution optical microscopy. Includes microfluidic sample cell. Microscope stage position is controlled in a feedback loop with position detection of trapped bead to run experiments under constant force.

Tags

Cell biophysics, Cytoskeleton, Light microscopy, Microtubuli, Optical Tweezers, Polymerisation forces

Application

Measurement of pN forces

References

No documentation available online

Location

E 0.18B

Specifications

Main application: Measurement of pN forces

Microscope:
Leica Inverted Microscope, 100x Objective lens
Transmitted light for Differential Interference Contrast (DIC) imaging

Light sources:
Trapping laser
Spectra-physics Millennia IR
Diode-pumped solid state laser
1064 nm, 7 W continuous wave
2 mm beam diameter Linear polarization

Detection laser
JDS Uniphase 1137/P
Helium-neon laser
632 nm 10mW continuous wave
Linear polarization

Other equipment:
Flow sensor Seyonic (micro-nano L volumes)
Home-built flow-cell
Pro Sense syringe pump

PI P-730AC nm-precision Piezo-stage xy, with manual micrometer actuators

Quadrant photodiode with home-built electronics
Kappa CCD camera (analogue) with DVD recorder

2 Acousto optic deflectors