Inverted confocal microscope setup. With capabilities for: deeper specimen penetration by using two photon imaging techniques, enhanced contrast by either scanning differential interference contrast (DIC) or phase contrast, epi-fluorescence. Max scanning speed 30 fps (512x512 pix) or higher, depending on the region of interest.

Confocal Microscopy

Microscope:
Nikon Ti eclipse Inverted microscope
Motorized non-encoded stage (XY-Translation)
Piezo Z-Stage (Z-translation)
Scanning DIC
Phase Contrast

Lasers:
Spectra Physics MAITAI eHPDS: Femtosecond pulsed tunable laser (70fs; 690-1040 nm), with dispersion correction.
Green Diode 561nm Laser (50mW) Sapphire.
ARGON LASER 65 mw MULTILINE (488nm, 514nm).

Other illumination sources:
Halogen Lamp 100W
Hg lamp

Detection:
A1R MP – Confocal Scanhead
4x PMT for fluorescence
Direct Illumination PMT for scanning DIC

Image Resolution:
Max 4096x4096 pixels (scan speed depends on image resolution)

Filters:
Epi-Fluorescence Filter Block FITC
Epi-Fluorescence Filter Block TRITC
A1 Filter Cube 525/50
A1 Filter Cube 595/50 700/75
A1 Filter Cube 540/30
A1-Filter Cube 450/50 IR CUT
A1-Filter Cube 482/35 IR CUT

E2.32b

Measured quantity: Quantitative: photon intensities (output: images).